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In the search for alternative production methods, various key intermediates or derivatives, such as hydrogenobyrate (HBA) 6 , hydrogenobyrinate a, c - diamide (HBAD) 7 and hydrogenobyric acid (Hby) 8 , have been obtained from engineered Eschericia coli strains, from which the titer of HBAD and Hby were reported to be 2.94 mg/L and 2.95 mg/L, respectively

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