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In bacteria, a single elongation factor is required to bind the SECIS element and the SeCys-tRNA, whereas in eukaryotes and archaea, those roles are filled by different proteins: SECIS-binding protein (SBP2), which appears to bind to the AUGA/GA stem nucleotides, and a SeCys-specific elongation factor (eEFSeCys) that partners with SBP2 for incorporation of UGA-encoded SeCys

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At the molecular level, studies indicate that GHK-Cu may interact with gene expression pathways involved in cellular differentiation, oxidative stress regulation, and matrix turnover