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this correlation may be attributed to the additional intestinal gas in patients with SIBO, causing an increase in intra-abdominal pressure, which could lead to a large reflux of gastric contents into the esophagus (Takakura and Pimentel, 2020)
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cDNA synthesis was performed using the High-Capacity Reverse Transcriptase Kit (Life Technologies) with random primers, following the manufacturer’s instructions for reactions without RNase inhibitor
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