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In Japan, ALS patients received B12 shots of 25,000-50,000mcg (each shot equal to 25-50 ‘pernicious anemia shots’ all at once), at first daily and then twice a week

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These results indicate that protein modification with the 3Br-5MP, along with reduction with NaBH 4 and oxidation with dehydrogenated ascorbic acid, was mild enough to produce active conjugates for biological applications