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Jan 2016;14(1):50-57
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The cells were left to differentiate into MDMs for 7 days, then washed with PBS, treated with 5 mM PBS/EDTA at 37ยฐC for 20 min, harvested gently with a cell scraper, counted and replated on 96- or 6-well trays at 1ร104 and 1ร106 cells per well, respectively, as described previously (31)
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