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These effects were inhibited by adrenomedullin receptor (ADMR) blockade, suggesting EV-derived adrenomedullin mediates these effects (216)
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The reaction was initiated by adding 4 ฮผL of 25 ฮผM stock purified NNMT protein (final concentration of NNMT in the reaction was 200 nM) and incubated on a heat block at 37ยฐ C
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