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Acetyl-CoA functions as a biosensor of the celluar metabolic state influencing specific genes in response to nutrient availability via epigenetic modificaitons such as histone acetylation ( NAD+ is a substrate of the glycolytic pathway and a crucial cofactor to mediate the deacetylation reactions catalyzed by the highly conserved sirtuin family of HDACs (78)

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They compared a target of 4.0 µg/day against typical European intakes which range from 4.2 to 8.6 μg/day in adults and believed 4.0 µg/day to be a practical amount