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To confirm that the phosphorylated MTHFR is less active than the non-phosphorylated form, under physiological SAM concentration conditions (1–3 μM), mutant MTHFR knock-in lines were created using the CRISPR method (Clustered Regularly Interspaced Short Palindromic Repeats)

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I paired the injection with the MIC+B12 and everything was uphill from there