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The needle passes through air containing bacteria, through the rubber which may have surface contaminants, and into a protein-rich solution that bacteria love to colonize
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This process is facilitated by the protonation of amine groups grafted onto the polymer surface within endosomes (pH 5–6) and lysosomes (pH 4–5), which possess strong proton-buffering capacity and induce rapid osmotic swelling, thereby promoting cargo release
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