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In the model of cyanobacterium Synechocystis PCC 6803, we showed that (i) MG is toxic, (ii) both the glxI and glxII genes are non-essential to cell life but are required for the protection against MG and (iii) the MG synthase (EC.4.2.99.11.) gene is also non-crucial to cell growth [61,214]
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TCE antibodies were added in triplicate and assay plates were returned to the incubator for two days, then read out by adding an equal volume of Steady-Glo (Promega) and reading out on a luminescence plate reader
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