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Lyophilization removes water, which slows many degradation pathways and makes the material easier to ship and store

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They were then lysed using RIPA lysis buffer containing 0.1% (w/v) SDS, 1% (w/v) Triton X-100, 150 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 50 mM Tris−HCl (pH 8), and protease inhibitor cocktail, with sonication