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Reported blood GSSG spans roughly 1 to more than 500 µmol/L, a spread attributable largely to method rather than biology (Rossi et al., 2002, PMID 11978601), and 5–15% of sample glutathione is artefactually oxidised during acid deproteination unless thiols are blocked first — preventable by N-ethylmaleimide derivatisation ahead of deproteination, with dichloromethane back-extraction (Giustarini et al., 2013, PMID 23928499)

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Treatment:12 Months

2014 doi: 10.1002/ptr.5232