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Once in the liver, both forms of vitamin D are hydroxylated by two key enzymes, CYP27A1 and CYP2R1, resulting in the production of 25-hydroxyvitamin D [25(OH)D], the primary circulating form and a reliable biomarker for assessing vitamin D status [17]

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Endogenous stimuli‐responsive systems exploit the unique biochemical signatures of diseased sites such as low pH, elevated levels of specific enzymes, and abnormal redox potential gradients, causing nanoparticle destabilization or conformational changes for the precise liberation of encapsulated redox cargo within the target tissue